From the Pirbright Institute:
The present invention provides a live, attenuated coronavirus comprising a variant replicase gene encoding polyproteins comprising a mutation in one or more of non-structural protein(s) (nsp)-10, nsp-14, nsp-15 or nsp-16. The coronavirus may be used as a vaccine for treating and/or preventing a disease, such as infectious bronchitis, in a subject.
FIELD OF THE INVENTION:
The present invention relates to an attenuated coronavirus comprising a variant replicase gene, which causes the virus to have reduced pathogenicity. The present invention also relates to the use of such a coronavirus in a vaccine to prevent and/or treat a disease.
SUMMARY OF ASPECTS OF THE INVENTION:
The present inventors have used a reverse genetics approach in order to rationally attenuate IBV. This approach is much more controllable than random attenuation following multiple passages in embryonated eggs because the position of each mutation is known and its effect on the virus, i.e. the reason for attenuation, can be derived.
Using their reverse genetics approach, the present inventors have identified various mutations which cause the virus to have reduced levels of pathogenicity. The levels of pathogenicity may be reduced such that when the virus is administered to an embryonated egg, it is capable of replicating without being pathogenic to the embryo. Such viruses may be suitable for in ovo vaccination, which is a significant advantage and has improvement over attenuated IBV vaccines produced following multiple passage in embryonated eggs.
Thus in a first aspect, the present invention provides a live, attenuated coronavirus comprising a variant replicase gene encoding polyproteins comprising a mutation in one or more of non-structural protein(s) (nsp)-10, nsp-14, nsp-15 or nsp-16.
The variant replicase gene may encode a protein comprising one or more amino acid mutations selected from the list of:
- Pro to Leu at position 85 of SEQ ID NO: 6,
- Val to Leu at position 393 of SEQ ID NO: 7;
- Leu to Ile at position 183 of SEQ ID NO: 8;
- Val to Ile at position 209 of SEQ ID NO: 9.
The replicase gene may encode a protein comprising the amino acid mutation Pro to Leu at position 85 of SEQ ID NO: 6.
The replicase gene may encode a protein comprising the amino acid mutations Val to Leu at position 393 of SEQ ID NO: 7; Leu to Ile at position 183 of SEQ ID NO: 8; and Val to Ile at position 209 of SEQ ID NO: 9.
The replicase gene may encodes a protein comprising the amino acid mutations Pro to Leu at position 85 of SEQ ID NO: 6; Val to Leu at position 393 of SEQ ID NO:7; Leu to Ile at position 183 of SEQ ID NO:8; and Val to Ile at position 209 of SEQ ID NO: 9.
h/t Hermit Sister
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The Pirbright Institute
The Pirbright Institute is a publicly funded private company. We have a diverse range of major stakeholders, and receive strategic funding from the Biotechnology and Biological Sciences Research Council (BBSRC) which is part of UK Research and Innovation (UKRI).
Our major stakeholders are:
- Biotechnology and Biological Sciences Research Council (BBSRC)
- UK Department for the Environment, Food and Rural Affairs (Defra)
- Wellcome Trust, Medical Research Council (MRC) and other research funding agencies
- International funding and disease control agencies, such as OIE, WHO, the European Commission, Bill and Melinda Gates Foundation
- Industrial producers of veterinary vaccines and antivirals
- Farmers and livestock keepers
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Bill Gates says vaccines can help reduce world population
The world today has 6.8 billion people… that’s headed up to about 9 billion. Now if we do a really great job on new vaccines, health care, reproductive health services, we could lower that by perhaps 10 or 15 percent.”
They always have to warn us ahead of time. April 27, 2018:
I doubt we are on the list of people whom they feel are worthy to live.
David DeGerolamo
When you see this and hear this it becomes obvious the spirits behind the scenes. They want our Trust they need us to seek them for their answers. Vaccines.
Mankind has meddled to far!
Trust in God, stop looking to man for ‘all’ the answers.
So many seem to seek mans answers first, then use God as a secondary back up system.
Keep in mind i say this, yet i did have a needed surgery this summer. i thanked the physicians and paid the hospital, Yet i praised and thanked God First and Foremost the entire time.
The fear mongering is the thing, they create the problem and then look at that , they too have the answer!
Run out and get your flu shots every single year now folks! Would not want you Trusting in a Higher power! How about shingles? Anything else we can provide you with for Free?
i do not trust Free!