The efficiency of delivery of DNA vaccines is often relatively low compared to protein vaccines. The use of superparamagnetic iron oxide nanoparticles (SPIONs) to deliver genes via magnetofection shows promise in improving the efficiency of gene delivery both in vitro and in vivo. In particular, the duration for gene transfection especially for in vitro application can be significantly reduced by magnetofection compared to the time required to achieve high gene transfection with standard protocols. SPIONs that have been rendered stable in physiological conditions can be used as both therapeutic and diagnostic agents due to their unique magnetic characteristics. Valuable features of iron oxide nanoparticles in bioapplications include a tight control over their size distribution, magnetic properties of these particles, and the ability to carry particular biomolecules to specific targets. The internalization and half-life of the particles within the body depend upon the method of synthesis. Numerous synthesis methods have been used to produce magnetic nanoparticles for bioapplications with different sizes and surface charges. The most common method for synthesizing nanometer-sized magnetite Fe3O4 particles in solution is by chemical coprecipitation of iron salts. The coprecipitation method is an effective technique for preparing a stable aqueous dispersions of iron oxide nanoparticles. We describe the production of Fe3O4-based SPIONs with high magnetization values (70 emu/g) under 15 kOe of the applied magnetic field at room temperature, with 0.01 emu/g remanence via a coprecipitation method in the presence of trisodium citrate as a stabilizer. Naked SPIONs often lack sufficient stability, hydrophilicity, and the capacity to be functionalized. In order to overcome these limitations, polycationic polymer was anchored on the surface of freshly prepared SPIONs by a direct electrostatic attraction between the negatively charged SPIONs (due to the presence of carboxylic groups) and the positively charged polymer. Polyethylenimine was chosen to modify the surface of SPIONs to assist the delivery of plasmid DNA into mammalian cells due to the polymer’s extensive buffering capacity through the “proton sponge” effect.
The date of the above publication is 2014. They have used their time well to refine the delivery process using protected mRNA to incorporate their “package” into your DNA. A pharmaceutical company that did not participate in Operation Warp Speed is predicting a 50% death rate in the first year and an 80% death rate within 5 years from this vaccine.
Joe Biden will deliver a speech Wednesday that will blame gun ownership for a dramatic rise in violent crime in America’s major cities.
White House press secretary Jen Psaki previewed Biden’s upcoming address during the daily press briefing on Monday.
“The President feels a lot — a great deal of the crime we’re seeing is a result of gun violence,” she said. “You can expect he’ll speak to that and his commitment to continuing to address gun violence and gun safety in the country.”
Biden will acknowledge the rise in violent crimes that occurred under his watch as Democrats face a crippling political narrative that their policies are to blame.
The murder rate has risen 25 percent and violent crime rose about three percent, according to new FBI data.
When Governor Roy Cooper first announced North Carolina’s Summer Cash and College Tuition drawings, specific groups of people were not immediately eligible based on where they got vaccinated.
That group included those vaccinated through the Veterans Administration (VA) among others. But that has since changed.
According to NCDHHS, those vaccinated by the Veterans Administration (VA) and through the Tribal Health Program are now eligible for the $4 Million Summer Cash and College Tuition drawings announced last week by Cooper to motivate people to get vaccinated as soon as they can.
Step right up and get your chance to win $1 million. All you have to do is get vaccinated with an untested mRNA cocktail that will rewrite your DNA to provide a continuous production of toxic COVID-19 spike proteins to attack your body’s organs.
For those who think this is not their fight, I disagree. Our enemy has stolen our government, our educational system, our health care, our Liberty and our wealth. They have marginalized our culture, religion and history. They have financed and supported the release of COVID-19 in order to vaccinate a majority of the world’s population with an untested mRNA vector which is now integrating into nuclear DNA. Once integrated, the production of the COVID spike protein will be continuous until death.
We have been sold out for money and for the reduction of the world’s population. For those who hoped that this day would not come, welcome to the jungle.
You can run and not look back. Or you can take a stand for your children. I do not believe there is anywhere safe to run so weigh your options carefully and be careful whom you trust.
A law signed by Florida Governor Ron DeSantis will allow for the Florida Department of Health to forcibly vaccinate Florida residents. S.B. 2006 was signed into law by DeSantis on May 3, 2021 and immediately hailed as a major victory by many medical freedom advocates. The law bans so-called “Vaccine Passports” and adds certain restrictions on state and local governments during a declared public health emergency. A little known and little spoken of section of the law, which will go into effect July 1, states: “The state health officer, upon declaration of a public health emergency, may take actions that are necessary to protect the public health.” Some of these actions include: “Ordering an individual to be examined, tested, vaccinated, treated, isolated, or quarantined for communicable diseases that have a significant morbidity or mortality and present a severe danger to public health.” Those refusing these actions “may be subjected to isolation or quarantine.” The most disturbing part is a section beneath which reads, “If the individual poses a danger to the public health, the State Health Officer may subject the individual to isolation or quarantine, if there is no practical method to isolate or quarantine the individual, the State Health Officer may use ANY MEANS NECESSARY to vaccinate or treat the individual.”